Properties of Cellulolytic Enzymes from Peel of Amorphophallus Paeoniifolius
نویسندگان
چکیده
Objective: Peel of Amorphophallus is considered as waste. In this study we checked for the presence of cellulase enzyme in this waste and investigated its biochemical properties. Methods: Presence of cellulase activity was confirmed by Congo red plate diffusion assay The optimum temperature, pH, substrate concentration for both CMCase and FPase activity was also determined by DNS method. Effect of salts and metal ions were also investigated. Zymography studies confirmed the presence of cellulase enzyme. Results: Optimum pH for CMCase was found to be 10.0 whereas pH 8 was optimum for FPase activity and optimum temperature was 60 °C .Hg2+ and Mn 2+ has strong inhibitory effect on cellulase activity and molecular weight of cellulase is around 43 kDa. Cellulase from peel of Amorphophallus was also found to be thermostable at 90oC for 1hour. Conclusion: Thermostable and pH stable cellulase from agrowaste can be of potential use in textile, dyeing industry.
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